Fig. 4. The expression of Peripheral CD4+ T cell subsets in control and achalasia group. (A) A CD3+CD8– gate used to identify CD4+ T cells. (B, C) Representative flow cytometric analysis of CD4 subsets, including T helper type 1 (Th1) cells, Th17 cells, pathological Th17 cells, Th2 cells, and T regulatory cells (Treg). (E-I) Scatter plot analysis comparing the proportion of CD4+ T cells across the individuals. Controls are shown in blue, and achalasia in red. P-values were calculated using t test or Wilcoxon test. *P < 0.05.
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