Fig. 3. The expression of CD8+ T cell and CD4+ T cells in peripheral and lower esophageal sphincter (LES) infiltration. (A) Zebra plot showed frequency of peripheral CD8+ T cells and CD4+ T cells measured by flow cytometry analysis. (B) Scatter plot showed analysis comparing the proportion of CD8+ T cells across the individuals. (C) Scatter plot showed analysis comparing the proportion of CD4+ T cells across the individuals. (D) Immunofluorescence staining of CD8+ T cells and CD4+ T cells in LES. (E) Number of CD8+ T cells per high-powered field (HPF) from LES. (F) Number of CD4+ T cells per HPF from LES. P-values were calculated using t test or Wilcoxon test. WBC, white blood cell. *P < 0.05.
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