Fig. 1. High-dimensional flow cytometry analysis of peripheral leukocytes in a learning cohort of achalasia. (A) The experimental workflow of peripheral blood sample collection and analysis. (B) Non-supervised and color-coded Uniform Manifold Approximation and Projection (UMAP) map depicting 10 clusters from 126 donors (achalasia, 76; control, 50). (C) Cell surface marker expression in the UMAP analysis. (D) UMAP map of all patients with controls (left) and achalasia (right). (E) Pie chart depicting the cellular composition of all leukocytes. (F) Stacked column graph of the frequencies of the 10 clusters in healthy controls and achalasia samples. RBC, red blood cell; NK, natural killer; mDC, classical dendritic cell; pDC, plasmacytoid dendritic cell.
© J Neurogastroenterol Motil
