Fig. 3. Electrophysiological responses (latency and amplitude of contractility) were obtained by stimulation of colon tissue circular strips. Control and infected groups were evaluated in the acute phase (AP; 11 days) and chronic phase (CP; 15 months post-infection [m.p.i.]) of Trypanosoma cruzi infection. Electrical stimuli of 0.5, 1, 5, 10, and 20 Hz were applied to colon tissue fragments in an organ bath system. (A and B) Latency in OFF-contractions. (C and D) Amplitude in OFF-contractions. (E and F) Latency in ON-contractions. n = 5, for each group. For (A) to (F), statistical analysis: two-way ANOVA and Duncan. Control (○); Infected (●). The symbols (*) represent differences in the relation to the group (control vs infected) and the letters (a, b, and c) represent the difference in the frequency, regarding the same group. aDifferent from 0.5 Hz. bDifferent from 1.0 Hz. cDifferent from 5.0 Hz. P ≤ 0.05. Data presented as mean ± SD. (G) Schematic drawing indicating the ON- and the OFF-latencies and the highest peak amplitude of the off contraction. CAP, control acute phase; IAP, infected acute phase; CCP, control chronic phase; ICP, infected chronic phase.
© J Neurogastroenterol Motil
