Fig. 2. Immunohistochemical expression of Toll-like receptor 4 (TLR4) in unaffected colonic mucosa of symptomatic uncomplicated diverticular disease (SUDD) patients and controls (control group [CG]). (A-B) TLR4 immunohistochemical expression does not show significant changes in SUDD patients (A) and in CG subjects (B), and involves scattered epithelial cells and inflammatory cells in lamina propria of the mucosa. (C) TLR4 expression was localized in epithelial cells (*) and endocrine-like cells (black arrows) present in the intestinal glands and in inflammatory cells (white arrows) in lamina propria. (D) Bars in the graphic represent the median average score of TLR4 immunohistochemical positivity calculated in 3 ×100 field for each sample using an ImageJ plugin. Box plots represent median, 25%-75% interquartile range and minimum-maximum values. Original magnification: ×100 (A-B), ×200 (C); high power field ×400. Scale bars, 250 µm (A-B), 25 µm (C).
© J Neurogastroenterol Motil
