Journal of Neurogastroenterology and Motility : eISSN 2093-0887 / pISSN 2093-0879

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Fig. 4. Inhibition of nitric oxide synthase (NOS) increases colonic contraction similarly in C57BL/10 and mdx mice. 4-week-old male C57BL/10 and mdx mice received either a control (Cont) diet or a diet supplemented with 1% L-alanine (Ala), L-citrulline (Cit), or L-arginine (Arg) for 8 weeks. After treatment, colons were excised, and contractions assessed ex vivo by video recording and spatiotemporal mapping of colon diameter in the presence of 100 μM N-nitro-L-arginine (NOLA), a NOS inhibitor (A). Contraction frequency (number of contractions per 15-minute recording) was determined for the proximal colon (B), the mid colon (C), and the distal colon (D). Statistical analysis was performed using a Kruskal-Wallis test to determine the effect of treatment. n = 9–12/genotype/group.
Journal of Neurogastroenterology and Motility 2020;26:133~146 https://doi.org/10.5056/jnm19029
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