J Neurogastroenterol Motil  
Piezo2: A Candidate Biomarker for Visceral Hypersensitivity in Irritable Bowel Syndrome?
Tao Bai, Ying Li, Jing Xia, Yudong Jiang, Lei Zhang, Huan Wang, Wei Qian, Jun Song,* and Xiaohua Hou
Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Correspondence to: Jun Song, MD
Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan 430022, China
Tel: +86-27-85726057, Fax: +86-27-85726057, E-mail: song111jun@126.com
Received: July 18, 2016; Revised: October 17, 2016; Accepted: October 23, 2016; Published online: January 2, 2017.
© The Korean Society of Neurogastroenterology and Motility. All rights reserved.

cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background/Aim
Currently, there exists no biomarker for visceral hypersensitivity in Irritable Bowel Syndrome (IBS). Piezo proteins have been proven to play an important role in the mechanical stimulation to induce visceral pain in other tissues and may also be a biomarker candidate. The aim of this study was to test the expressions of Piezo1 and Piezo2 proteins in the intestinal epithelial cells from different intestinal segments and to explore the correlation between Piezo proteins expression and visceral pain threshold.
Methods
Post-infectious IBS was induced in mice via a Trichinella spiralis infection. Visceral sensitivity was measured with abdominal withdrawal reflex to colorectal distention. Inflammation in the small intestine and colon was scored with H&E staining. Expression location of Piezo proteins was confirmed by immunohistochemistry. Abundance of Piezo proteins were measured with real-time reverse transcriptase polymerase chain reaction.
Results
Piezo1 and Piezo2 proteins were expressed in the intestinal epithelial cells. The expression levels of Piezo1 and Piezo2 were abundant in the colon than the small intestine (P < 0.05). Expression of Piezo2 in the colon significantly correlated to the visceral sensitivity (r = -0.718, P < 0.01) rather than the mucosal inflammation.
Conclusion
Piezo2 is a candidate biomarker for visceral hypersensitivity in IBS.
Keywords: Hyperalgesia; Irritable bowel syndrome; Ion channels; Pizeo1 protein, human; Piezo2 protein, human


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