Journal of Neurogastroenterology and Motility : eISSN 2093-0887 / pISSN 2093-0879

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Fig. 1. DNA methyltransferase (DNMT) expression levels in intestinal tissue from mice and humans. (A) Using the Smooth Muscle Transcriptome Browser, we show the expression levels of various DNMTs in several intestinal cell types and tissues (J, jejunal; C, colonic; SM, smooth muscle tissue; SMC, smooth muscle cell; ICC, interstitial cells of Cajal; PαC, platelet-derived growth factor receptor α-positive [PDGFRα+] cell; Mu, mucosa tissue; and MuPαC, mucosal PDGFRα+ cell). Dnmt3a is the most highly expressed Dnmt isoform in colonic and jejunal smooth muscle tissue but this pattern is not consistent amongst all isolated cell types as JPαC/CPαC/CMu/CMuPαC express Dnmt1 more than Dnmt3a with Dnmt3b consistently being expressed the least amongst all cell types and tissues. While these expression levels are informative, they do not indicate necessity as Dnmt1 knockout causes the most detrimental phenotype in both intestinal epithelia and smooth muscle. (B) Expression levels of DNMT and 10–11 traslocation (TET) proteins in mice reveal that DNMT1 reduces its expression over time with a opposite pattern for DNMT3A (Adapted from Jorgensen et al). (C) Variously diseased human tissue shows a dysregulation of DNMT1 and TET3 while other enzymes remain relatively stable in expression across conditions (Adapted from Jorgensen et al). FPKM, Fragments Per Kilobase Million; UBE, Ubiquitin-activating enzyme; M, marginal area; P, pouch.
Journal of Neurogastroenterology and Motility 2019;25:377~386
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