Journal of Neurogastroenterology and Motility : eISSN 2093-0887 / pISSN 2093-0879

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Fig. 4. Influence of MKC-231, a choline uptake enhancer, on high-affinity choline transporter 1 (CHT1) expression and intestinal motility in vivo. (A) Immunoblots (left) and histogram (right) for CHT1 in the colon from control and MKC-231-treated rats showing that the ratios of CHT1 to β-actin expression in control rats are significantly increased after 10 consecutive days of MKC-231 treatment. (B) Merged pictures of immunostaining for CHT1, transmembrane member 16A (TMEM16A), and DAPI (4′,6-diamidino-2-phenylindole) in longitudinal colonic sections from control (left) and MKC-231 rats (right) (×400) revealing that the density of CHT1 labelling in the colonic muscular layer is considerably elevated after treatment with MKC-231. White arrows indicate CHT1-positive intramuscular interstitial cells of Cajal. White arrowheads indicate CHT1-immunoreactive (IR) neurons in the myenteric plexus. (C) The intestinal transit time of MKC-231-rats is reduced compared to that of control rats. (D) The fecal water content of the MKC-231 group is considerably increased compared with that of the control group. *P < 0.05 compared to controls.
Journal of Neurogastroenterology and Motility 2018;24:643~655
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